Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway

Fig. 3

MSC-Mito and PQQ synergistically promote mitochondrial biogenesis while inhibiting DNA damage and cellular apoptosis. (A) Western blot analysis of SIRT1 and PGC-1α protein expression in mouse ovarian tissues of various groups. (B) Western blot analysis of pATM and TERF2 protein expression in mouse ovarian tissues of various groups. (C) Western blot analysis of the apoptosis-related proteins p53, Bax, and Bcl-2 expression in mouse ovarian tissues of various groups. (D) Western blot analysis of SIRT1 and PGC-1α protein expression in KGN cells. (E-F) qPCR analysis of SIRT1 and PGC-1α gene expression. (G) qPCR analysis of mtDNA copy number. (H-I) Comet assay to assess DNA damage. (J-K) Flow cytometry analysis to detect cellular apoptosis. (L) Western blot analysis of the impact of PQQ and MSC-Mito on the ATM/p53 signalling pathway in vitro. Scale bars: 10 μm. Values are the means ± SEMs. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512