Manufacturing, quality control, and GLP-grade preclinical study of nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles

Table 2 List of top 20 known microRNAs detected in 3 lots of haMSC-EVs

Rank	microRNA	Relative content (TPM) × 10⁴			CV (%)
Rank	microRNA	Lot1	Lot2	Lot3	CV (%)
1	hsa-let-7b-5p	15.31	13.54	12.89	9
2	hsa-let-7a-5p	14.99	10.97	14.28	16
3	hsa-miR-1290	10.89	11.72	9.90	8
4	hsa-miR-126-5p	7.03	5.43	8.78	24
5	hsa-miR-23a-3p	4.82	4.42	6.43	20
6	hsa-let-7d-3p	4.33	5.38	5.82	15
7	hsa-let-7f-5p	3.47	2.27	3.56	23
8	hsa-miR-92a-3p	2.42	2.56	1.71	20
9	hsa-let-7c-5p	2.30	2.27	1.76	14
10	hsa-miR-574-3p	2.05	1.54	2.13	17
11	hsa-miR-25-3p	1.63	2.23	1.83	16
12	hsa-miR-151a-3p	1.48	1.12	1.52	16
13	hsa-miR-1246	1.65	1.24	1.00	26
14	hsa-miR-21-5p	1.49	0.88	1.27	26
15	hsa-miR-146a-5p	0.97	1.26	0.92	18
16	hsa-miR-24-3p	0.88	1.11	1.03	12
17	hsa-let-7e-5p	1.01	1.18	0.74	22
18	hsa-let-7b-3p	0.99	0.81	0.94	10
19	hsa-miR-1260a	0.62	1.00	0.89	23
20	hsa-miR-1260b	0.55	0.95	0.75	27

ISSN: 1757-6512