Manufacturing, quality control, and GLP-grade preclinical study of nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles

Wang, Jing; Chen, Zhong-jin; Zhang, Ze-yi; Shen, Mei-ping; Zhao, Bo; Zhang, Wei; Zhang, Ye; Lei, Ji-gang; Ren, Cheng-jie; Chang, Jing; Xu, Cui-li; Li, Meng; Pi, Yang-yang; Lu, Tian-lun; Dai, Cheng-xiang; Li, Su-ke; Li, Ping

doi:10.1186/s13287-024-03708-1

Stem Cell Research & Therapy

Table 3 “Exosomal markers” reported in ExoCarta identified in 3 lots of haMSC-EVs

From: Manufacturing, quality control, and GLP-grade preclinical study of nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles

Number	Gene symbol	Gene name	Peptide number identified in haMSC-EVs	Number of times identified in ExoCarta database
1	FLNA	Filamin-A	85	56
2	THBS1	Thrombospondin-1	76	46
3	A2M	Alpha-2-macroglobulin	74	54
4	ALB	Albumin	60	63
5	MYH9	Myosin-9	54	44
6	CLTC	Clathrin heavy chain	41	64
7	HSPA8	Heat shock cognate 71 kDa protein	37	96
8	VCP	Transitional endoplasmic reticulum ATPase	29	62
9	ACTB	Actin, cytoplasmic 1	28	93
10	ACTN4	Alpha-actinin-4	27	51
11	PKM	Pyruvate kinase PKM	27	72
12	PTGFRN	PTGFRN protein	26	46
13	MSN	Moesin	23	62
14	PGK1	Phosphoglycerate kinase 1	23	69
15	HSPA5	Endoplasmic reticulum chaperone BiP	22	58
16	ANXA6	Annexin A6	22	49
17	ALDOA	Fructose-bisphosphate aldolase	20	69
18	ACLY	ATP-citrate synthase	19	48
19	ANXA2	Annexin A2	17	83
20	ATP1A1	Sodium/potassium-transporting ATPase subunit alpha	17	57
21	HSP90AA1	Heat shock protein HSP 90-alpha	17	77
22	LGALS3BP	Galectin-3-binding protein	17	53
23	MVP	Major vault protein	17	44
24	ENO1	Alpha-enolase	16	78
25	EEF2	Elongation factor 2	15	69
26	FASN	Fatty acid synthase	15	66
27	ITGA6	Integrin alpha-6	15	45
28	ITGB1	Integrin beta	15	60
29	UBA1	Ubiquitin-like modifier-activating enzyme 1	15	45
30	RAP1B	Ras-related protein Rap-1b	14	60
31	KPNB1	Importin subunit beta-1	14	48
32	TUBA1C	Tubulin alpha chain	14	47
33	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	14	95
34	MFGE8	Lactadherin	14	52
35	RAB1A	Ras-related protein Rab-1A	13	45
36	ANXA5	Annexin A5	13	67
37	EEF1A1	Elongation factor 1-alpha	12	71
38	TCP1	T-complex protein 1 subunit alpha	11	44
39	CCT5	CCT-epsilon	11	46
40	HSP90AB1	Heat shock protein HSP 90-beta	11	67
41	RAB8A	Ras-related protein Rab-8A	11	44
42	CCT2	T-complex protein 1 subunit beta	10	56
43	CCT3	T-complex protein 1 subunit gamma (Fragment)	10	46
44	GNAI2	Guanine nucleotide-binding protein G(i) subunit alpha-2	10	53
45	RAB7A	Ras-related protein Rab-7a	10	50
46	STOM	Erythrocyte band 7 integral membrane protein	10	44
47	YWHAZ	14–3-3 protein zeta/delta	10	69
48	CFL1	Cofilin, non-muscle isoform	9	62
49	EZR	Ezrin	9	48
50	LDHB	L-lactate dehydrogenase A chain	9	50
51	YWHAB	14–3-3 protein beta/alpha	9	50
52	LDHA	L-lactate dehydrogenase	9	72
53	SLC3A2	4F2 cell-surface antigen heavy chain	9	57
54	TPI1	Triosephosphate isomerase	9	62
55	PPIA	Peptidyl-prolyl cis–trans isomerase	8	62
56	CD9	Tetraspanin	8	98
57	RAB14	Ras-related protein Rab-14	8	47
58	TFRC	Transferrin receptor protein 1	8	47
59	YWHAQ	14–3-3 protein theta	8	56
60	PFN1	Profilin-1	8	61
61	EHD4	EH domain-containing protein 4	7	51
62	GNB1	guanine nucleotide binding protein (G protein), beta polypeptide 1	7	47
63	GNB2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	7	57
64	RAC1	Ras-related C3 botulinum toxin substrate	7	53
65	RAN	GTP-binding nuclear protein Ran	7	46
66	YWHAH	14–3-3 protein eta	7	44
67	PRDX1	Peroxiredoxin-1	7	61
68	CDC42	Cell division control protein 42 homolog	6	55
69	TKT	Transketolase	6	44
70	YWHAG	14–3-3 protein gamma	6	54
71	GNAS	GNAS complex locus isoform 2	5	50
72	RAB5C	Ras-related protein Rab-5C	5	49
73	SDCBP	Syndecan binding protein (Syntenin), isoform CRA_a	5	78
74	AHCY	Adenosylhomocysteinase	4	46
75	RHOA	Transforming protein RhoA	4	52
76	CD63	Tetraspanin	4	82
77	BSG	Basigin	3	45
78	CD81	CD81 antigen	3	64
79	RAB5B	Ras-related protein Rab-5B	3	45
80	LAMP2	Lysosome-associated membrane glycoprotein 2	1	45
81	PRDX2	cDNA FLJ60461, highly similar to Peroxiredoxin-2	1	51

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ISSN: 1757-6512

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