Skip to main content

Table 6 Effects of haMSC-EVs on respiratory function in rats

From: Manufacturing, quality control, and GLP-grade preclinical study of nebulized allogenic adipose mesenchymal stromal cells-derived extracellular vesicles

Genders

Groups

Indicators

Before administration

After administration

15 min

30 min

45 min

1 h

2 h

4 h

8 h

24 h

Female

Control

TV (mL)

0.92 ± 0.06

1.18 ± 0.10**

1.11 ± 0.10**

0.99 ± 0.03

0.98 ± 0.04

1.05 ± 0.12

1.01 ± 0.07

1.10 ± 0.10**

1.07 ± 0.08*

MV (mL)

161.4 ± 96.28

159.8 ± 26.58

109.4 ± 15.06

133.6 ± 53.28

121.4 ± 18.89

107.4 ± 11.91

119.0 ± 35.27

126.0 ± 25.15

147.0 ± 70.04

RR (bpm)

192.0 ± 126.68

144.6 ± 25.81

101.8 ± 20.41

147.2 ± 67.28

133.8 ± 25.31

108.2 ± 14.27

126.4 ± 51.64

122.4 ± 31.00

140.8 ± 67.45

haMSC-EVs-low

TV (mL)

0.97 ± 0.05

1.16 ± 0.11**

1.04 ± 0.07

0.92 ± 0.09

0.98 ± 0.04

1.00 ± 0.08

1.05 ± 0.04

1.07 ± 0.07

1.06 ± 0.12

MV (mL)

119.8 ± 31.45

151.6 ± 32.46

113.8 ± 20.4

112.4 ± 46.69

109.0 ± 18.28

124.8 ± 33.91

124.6 ± 32.05

139.6 ± 50.09

167.2 ± 55.37

RR (bpm)

133.00 ± 45.01

146.00 ± 41.03

113.20 ± 25.33

136.60 ± 89.70

115.60 ± 29.36

134.60 ± 51.00

126.00 ± 37.28

142.40 ± 52.30

175.00 ± 80.83

haMSC-EVs-medium

TV (mL)

0.99 ± 0.04

15.41 ± 31.98

1.11 ± 0.17

1.00 ± 0.13

0.99 ± 0.06

1.00 ± 0.14

1.09 ± 0.07

1.06 ± 0.07

1.13 ± 0.11

MV (mL)

165.60 ± 64.79

506.40 ± 837.46

126.00 ± 29.35

127.00 ± 38.44

134.80 ± 36.09

113.80 ± 35.65

143.40 ± 51.56

117.60 ± 27.90

149.40 ± 23.80

RR (bpm)

183.80 ± 74.88

128.80 ± 24.99

123.00 ± 41.58

134.40 ± 59.86

144.20 ± 41.58

117.20 ± 27.33

141.00 ± 54.41

117.40 ± 27.18

147.60 ± 37.55

haMSC-EVs-high

TV (mL)

0.96 ± 0.08

1.22 ± 0.11**

1.10 ± 0.11

0.96 ± 0.07

0.99 ± 0.09

1.03 ± 0.11

1.04 ± 0.11

1.06 ± 0.11

1.15 ± 0.10*

MV (mL)

135.80 ± 60.32

141.40 ± 11.99

120.60 ± 31.37

139.40 ± 67.88

127.00 ± 32.72

108.20 ± 15.25

107.20 ± 14.69

129.20 ± 40.53

136.20 ± 33.41

RR (bpm)

155.00 ± 78.58

124.00 ± 23.57

113.80 ± 33.08

162.00 ± 89.15

136.40 ± 31.69

111.80 ± 30.96

110.20 ± 21.75

130.80 ± 42.61

127.60 ± 35.86

Male

Control

TV (mL)

0.96 ± 0.15

1.11 ± 0.17

1.08 ± 0.21

1.03 ± 0.18

1.02 ± 0.18

0.99 ± 0.15

1.07 ± 0.13

1.05 ± 0.16

1.06 ± 0.13

MV (mL)

129.00 ± 35.92

138.60 ± 17.62

133.00 ± 30.14

115.80 ± 23.61

123.00 ± 26.18

106.80 ± 11.10

131.80 ± 30.41

129.40 ± 39.76

123.00 ± 7.28

RR (bpm)

144.20 ± 31.07

132.00 ± 18.88

132.20 ± 20.12

115.60 ± 18.72

129.00 ± 41.18

113.20 ± 14.29

131.40 ± 25.31

128.20 ± 31.00

119.80 ± 13.20

haMSC-EVs-low

TV (mL)

0.86 ± 0.05

1.17 ± 0.11**

1.02 ± 0.08**

0.92 ± 0.11

0.93 ± 0.06

0.91 ± 0.10

0.91 ± 0.10

0.97 ± 0.08

0.92 ± 0.11

MV (mL)

144.40 ± 83.16

138.60 ± 15.36

121.60 ± 22.21

105.80 ± 6.83

147.80 ± 30.29

119.00 ± 32.28

132.60 ± 55.08

144.60 ± 34.82

113.20 ± 13.52

RR (bpm)

176.00 ± 105.08

124.60 ± 11.44

122.60 ± 28.45

115.60 ± 7.77

166.80 ± 32.27

131.20 ± 20.18

155.60 ± 60.31

166.60 ± 50.59

126.60 ± 7.02

haMSC-EVs-medium

TV (mL)

0.97 ± 0.09

1.18 ± 0.14

1.12 ± 0.14

1.06 ± 0.10

1.05 ± 0.14

0.94 ± 0.07

1.04 ± 0.08

1.04 ± 0.17

1.06 ± 0.12

MV (mL)

150.00 ± 51.04

145.20 ± 20.71

136.40 ± 49.54

110.20 ± 9.09

115.00 ± 6.60

119.60 ± 20.56

116.80 ± 16.30

139.40 ± 27.33

131.40 ± 29.81

 

RR (bpm)

168.80 ± 69.03

125.40 ± 19.24

118.60 ± 29.60

105.20 ± 11.12

111.20 ± 13.31

133.20 ± 32.92

117.80 ± 21.92

147.80 ± 49.94

124.40 ± 16.10

haMSC-EVs-high

TV (mL)

0.87 ± 0.06

1.10 ± 0.12*

1.01 ± 0.14

0.94 ± 0.09

0.91 ± 0.07

0.90 ± 0.08

1.04 ± 0.07

0.96 ± 0.09

1.03 ± 0.12

MV (mL)

143.20 ± 60.35

141.00 ± 37.66

123.00 ± 20.31

116.80 ± 24.18

113.80 ± 24.20

121.80 ± 22.40

127.20 ± 15.45

121.00 ± 14.75

146.80 ± 38.44

RR (bpm)

170.40 ± 63.74

127.80 ± 24.24

124.00 ± 13.40

128.60 ± 26.02

130.20 ± 26.76

143.80 ± 32.28

127.80 ± 13.10

134.60 ± 33.19

147.00 ± 32.75

  1. TV, tidal volume; MV, minute volume; RR, respiratory rate. Data were expressed as mean±SD, N=5. * and ** indicate significantly changed after administration (* P ≤0.05, ** P ≤0.01)
Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512