Fig. 2

Transplantation of iMNP in contusive chronic SCI model. a Representative images of Ki-67 positive proliferating cells in each milestone cell type and proliferation were analyzed using the Cell Counting Kit-8 assay. b Experimental scheme of generation of contusive chronic SCI rat model. c The transplanted iMNPs show white and gray matter at the lesion epicenter. d IF stained image of OLIG2 and iMNP in the dorsal portion of the lesional site. e IF stained image of ChAT and iMNP in the gray matter of the lesional site. f Expression of SMI-32 in the transplanted iMNP cells was mostly noted around the ependymal cell layer and posterior part of the lesion. g BBB scores improved at 12 and 14 weeks after SCI (PBS, n = 4; 12 weeks iMNP, n = 3, and 14 weeks iMNP n = 2). Data are presented as mean ± SEM. Scale bars = 50 and 20 μm. iMNP, induced pluripotent stem cell-derived motor neuron progenitor cells; SCI, spinal cord injury; IF, immunofluorescence; ChAT, choline acetyltransferase; BBB, Basso–Beattie–Bresnahan