Table 1 List of Antibodies, Host, and Dilutions information

Motor neuron differentiation

SOX1

Goat

1:50 †

R&D SYSTEMS

OLIG2

Rabbit

1:50 †

Millipore

1:700 *

ChAT

Goat

1:50 †

Millipore

1:700 *

HB9

Rabbit

1:50 †

Millipore

SMI-32

Mouse

1:50 †

BioLegend

1:700 *

Cell proliferation

Ki-67

Goat

1:50 †

Santa cruz

Neuron, Astrocyte, Reactive Astrocyte and Oligodendrocyte

Neu N (Neuron)

Mouse

1:100 †

Abcam

1:700 *

GFAP (Astrocyte)

Mouse

1:100 †

Merck

1:700 *

Rabbit

1:1000 †

Abcam

1:10000 *

C3

(Reactive astrocyte, A1)

Rabbit

1:500 †

Abcam

1:1000 *

CC-1 (Oligodendrocyte)

Mouse

1:100 †

Merck

1:700 *

Growth factors

NGF

Rabbit

1:50 †

Abcam

1:700 *

BDNF

Rabbit

1:50 †

GeneTex

1:700 *

VEGF

Mouse

1:50 †

Santa cruz

1:700 *

Synaptogenesis and Axonal regeneration

Synapsin-1

Rabbit

1:50 †

Sigma-Aldrich

1:700 *

MAP-2

Mouse

1:50 †

Santa cruz

1:700 *

House Keeping Protein

GAPDH

Mouse

1:2000 *

Abcam

Beta actin

Rabbit

1:2000 *

Abcam