Fig. 3

Echocardiographic analysis of systolic and diastolic heart function of mouse diabetes model experiments. A. Representative examples of 2D echocardiography images from the left parasternal long-axis view (upper) and the apical four-chamber view (lower) in three groups of experimental mice. B-E. Graphs of echocardiographic indices of systolic function - EF (B), FS (C); and diastolic function - E/A (D) and IVRT (E) in three groups of animals each from experiments of 10 weeks and 18 weeks duration. n = 6 per group for all experiments. Results presented as mean ± SD. Statistical analysis was performed by 2-way Analysis of Variance (ANOVA) with adjustment for multiple comparisons. *:P < 0.05; **:P < 0.01; ***:P < 0.001; ****:P < 0.0001. Abbreviations: Non-DM = non-diabetic group; DM = diabetes mellitus with injection of saline; DM + MSC = diabetes mellitus with injection of hUC-MSCs; EF = ejection fraction; FS = fractional shortening; E/A = E/A ratio; IVRT = isovolumic relaxation time