Fig. 5

Analyses of micro RNAs and messenger RNAs in cardiac tissue from diabetes model experiments: A. Heatmap, derived from publically available dataset GSE210036 of the top 50 differentially expressed miRNAs in hearts of mice with diabetic cardiomyopathy (DCM) to those of mice without DCM (Non-DCM). B-C. Graphs of quantitative RT-PCR analyses of the relative expression of microRNA-133a in heart tissue of three groups of mice each from experiments of 10 weeks (B) and 18 weeks (C) duration. Levels for DM and DM + MSC groups are expressed as fold change relative to the Non-DM group. D. Volcano plot derived from publically-available dataset GSE161052 of differentially expressed mRNAs in hearts of mice with DM compared to those without DM. Annotations indicate the gene names for the top 18 differentially expressed mRNAs (red = higher in DM, blue = lower in DM). * = miRNAs and mRNAs selected for validation in the current study. E. The binding site of Col1A1 and miR-133a from website TargetScan. F-G. Graphs of quantitative RT-PCR analyses of the relative expression of mRNAs COL1A1 and COL3A1 in heart tissue of three groups of mice each from experiments of 10 weeks (F) and 18 weeks (G) duration. Levels for DM and DM + MSC groups are expressed as fold change relative to the Non-DM group. H-I. Graphs of quantitative RT-PCR analyses of the relative expression of six selected mRNAs related to fibrosis in heart tissue of three groups of mice each from experiments of 10 weeks (H) and 18 weeks (L) duration. Levels for DM and DM + MSC groups are expressed as fold change relative to the Non-DM group. For B, C, F, G, H, and G, all values are expressed as mean ± SD of n = 6 biological replicates. Statistical analysis was performed by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, respectively. Abbreviations: Non-DM = non-diabetic group; DM = diabetes mellitus with injection of saline; DM + MSC = diabetes mellitus with injection of hUC-MSCs