Fig. 5

Changes in expression of KRT19 ⁺ epithelial cells and their ligand and receptor pairs after different treatments. (A) Representative immunohistochemical images of KRT19 ⁺ epithelial cells after different treatments (4-weeks post-injection) for OSF. The black boxes represent the locally enlarged position. Scale bars = 100 μm, 50 μm. (B) Quantitative analysis of the expression of KRT19 ⁺ epithelial cells in (A), n = 6. (C) Representative images of KRT19 ⁺ MIF ⁺ epithelial cells after different treatments (4-weeks. post-injection) for OSF. Scale bar = 50 μm. (D) Representative images of immunostaining for CD74 ⁺ and CXCR4 ⁺ T cells under different treatments (4-weeks post-injection) for OSF. The red arrows indicate the positive cells. Scale bar = 50 μm. (E&F) Semiquantitative analysis of the percentage of the fluorescence area in Merge was performed according to the results in (C&D), respectively, n = 6. (G)&(H) Representative immunofluorescence images of Epi1.2 cells cocultured with T cells and DPSC-CM in vitro, n = 3. Scale bars = 50 μm. (I)&(J) Semiquantitative analysis of the fluorescence area in Merge, performed according to the results in (G&H), respectively, n = 3. The results are presented as the mean ± S.D. *P < 0.05; ***P < 0.001; ****P < 0.0001