Table 2 Haploid cells for genetic screening
From: Development and application of haploid embryonic stem cells
Cell type | Mutant method | Target gene | Target gene function | reference |
|---|---|---|---|---|
Mouse haESCs | retrovirus | Gpr 107 | Ricin toxicity | Elling, U., et al. [6] |
Mouse haESCs | retrovirus | SPEN | X-chromosome inactivation | Monfort, A., et al. [26] |
Human haESCs | retrovirus | NUDT5 | Resistance to 6-TG | Sagi, I., et al. [13] |
Mouse haESCs | PB | Msh2, Hprt | Resistance to 6-TG | Leeb, M. and A. Wutz [7] |
Mouse haESCs | PB | Parp1 | Resistance to olaparib | Pettitt, S.J., et al. [27] |
Monkey haESCs | PB | PRKD1 et al. | Detection of mutation efficiency | Yang, H., et al. [10] |
Mouse haESCs | PB | 2fp706, Pum1 | Exit from self-renewal | Leeb, M., et al. [28] |
Mouse haNSCLSs | PB | Park2 | Mn2+ toxicity | He, Z.Q., et al. [29] |
Monkey haNPCs | PB | B4GALT6 | Tetrodotoxin-like toxicant | Wang, H., et al. [30] |
Mouse haiTSCs | PB | Htra1 | Blocker for spongiotrophoblast specification | Peng, K., et al. [31] |
Mouse haTSCs | PB | Hprt | Resistance to 6-TG | Cui, T., et al. [32] |
Rat haESCs | PB | Thop1 | Related to pluripotency | Xu, M., et al. [22] |
Mouse haESCs | PB | Slc25a43 | Oxidative toxicity | Zhang, J., et al. [23] |
Mouse haEpiSCs | PB | Hs3St3b1 | Related to reprogramming | Gao, Q., et al. [24] |
Mouse haESCs | EMS | Hprt | Resistance to 6-TG | Forment, J.V., et al. [33] |
Mouse haESCs | CRISPR/Cas 9 | Zicl, Clec11a, et al. | Related to bone development | Bai, M., et al. [34] |
Mouse haESCs | CRISPR/Cas 9 | 4 amino acids of DND1 | Related to stability of DND1 protein | Li, Q., et al. [35] |
Human haESCs | CRISPR/Cas 9 | ATF7IP | Related to maternally imprinted | Bar, S., et al. [25] |
Human haESCs | CRISPR/Cas 9 | p53 | resistance to a large array of anticancer drugs | Segal, E., et al. [36] |
Human haESCs | CRISPR/Cas 9 | BRCA2 | sensibility to PARP inhibitors | Li, H., et al. [37] |
Human haNSCs | PB | SHANK2, KMT5B, et al. | manganese-induced toxicity genes | Wang, HS., et al. [21] |
Mouse haESC | PB | Catip, Dyrk1a | Conversion from ESC to TSC | Zhang, W., et al. [38] |