Fig. 2

Secretome improves renal function following bilateral I/R injury. Plasma creatinine levels were measured longitudinally during a 5 day protocol following either sham surgery or post IRI injury. A Plasma creatinine 24 h post I/R prior to randomization of rats into respective treatments (Sham: n = 7; Vehicle: n = 16; and ASC-CS: n = 14) (B) Rats received two injections of either secretome (2 mg/kg; 1 ml) or saline (vehicle; 1 ml) via subcutaneous injection after 24 h and 72 h post IRI (arrows) following collection of blood for renal functional assessment. Change of plasma creatinine levels over time in rats with initial creatinine values of 3.0 mg/dl or greater (C) or in rats with creatinine values 2.9 mg/dl or lower (D). Data are presented as mean ± SEM. *indicates P < 0.05 vs in ASC-CS vs vehicle treated animals by repeated measures ANOVA and Tukey’s post-hoc test