Figure 3
From: Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration
Effects of adipose stem cell (ASC)-conditioned medium on chondrocyte phenotype, proliferation, apoptosis, and angiogensis. (A) Diagram of ASC-conditioned media experiments, (B) [35S]-sulfate incorporation, (C) alkaline phosphatase activity, (D) [3H]-thymidine incorporation, (E) caspase-3 activity, (F) bax/bcl2 mRNA levels, (G) DNA fragmentation, and (H) endothelial tube length. All experiments were assayed after 24 hours of conditioned media treatment except for bax/bcl2 mRNA levels and endothelial tube length, which were measured after 12 hours of treatment. X-axis labels refer to treatments and culture type for chondrocytes (Chond), ASCs, and liver cells (Liv) prior to conditioning, which include growth medium (GM), chondrogenic medium (CM), monolayer (ML), and microbead (μB) (n = 6 ± SE). *P < 0.05 vs. control; #P < 0.05 vs. ASCs; ^P < 0.05 vs. ASC microbeads (ASCs + μB).