From cytogenomic to epigenomic profiles: monitoring the biologic behavior of in vitro cultured human bone marrow mesenchymal stem cells

Table 2 Conventional cytogenetic analysis on hBM-MSCs from eight healthy donors at several passages in vitro

Donor	Passage	Norm. karyotype (%)	Clonal aneupl. (%)	Random loss (%)	Random gain (%)	Metaphases (number)	Metaphases/Cells (number)	Mitotic index (%)
1 (46,XX)	7	45.5	0	54.5	0	22	24/1038	2.3
	9	66.7	0	33	0	39	8/1000	0.8
	12^a	60	0	40	0	5	15/1000	1.5
2 (46,XY)	6	82.8	0	15.6	1.6	64	70/1000	7.0
	10	80	0	20	0	30	24/1010	2.4
	16^a	45.5	0	36.4	18.1	11	11/1007	1.1
3 (46,XX)	3	70	0	26	4	23	4/1032	0.4
4 (46,XX)	3	78	0	22	0	27	16/1000	1.6
	6	70.4	0	25.9	3.7	27	16/1000	1.6
	9	41.2	52.9 (47,XX,+7)	5.9	0	17	2/1320	0.2
	12	30.6	50 (47,XX,+7) and 11.1 (46,X,+7)	8.3	0	36	16/1000	1.6
5 (46,XX)	4	33.3	33.3 (49,XX,+5,+7,+9)	33.3	0	18	12/1011	1.2
	6	80	0	12	8	25	7/1000	0.7
	10	81.3	0	18.7	0	32	14/1080	1.3
	12	92.3	0	7.7	0	26	18/1028	1.8
6 (46,XY)	4	86	0	14	0	50	47/1000	4.7
	6	76.5	0	23.5	0	17	2/1001	0.2
	9	82.7	0	17.3	0	53	41/1000	4.1
	12^a	72.7	0	18.2	9.1	11	1/1000	0.1
7 (46,XX)	4	90.6	0	3.1	6.3	32	49/1011	4.8
	6	92.9	0	7.1	0	14	2/1000	0.2
	9	81.3	0	18.7	0	16	11/1029	1.1
8 (46,XY)	4	100	0	0	0	37	27/1030	2.6
	6	78.1	0	17	4.9	41	22/1088	2.0
	9	77	0	23	0	30	6/1000	0.6

^aIn senescence. Clonal aneupl., clonal aneuploidies; Struct. abnorm., structural abnormalities.

ISSN: 1757-6512