Figure 4

The potent myotube-forming capacity of WT MDSCs in myogenic medium is decreased but still maintained under high serum concentrations, in the presence of steady MyoD expression. (A) Representative micrographs of myotubes generated in confluent WT MDSCs maintained for 2 weeks in myogenic medium, as evidenced by immunocytochemistry for MHC II (200×); (B) and (C) as (A), but in PM with 20% or 10% serum (200×). (D) Representative Western blots for WT MDSCs incubated in triplicate in each of the previously mentioned cultures in the three types of media subjected to immunodetection for MHC II (210 kDa) and MyoD (44 kDa). 10, 10% PM; 20, 20% PM; H, myogenic medium; WT MDSC, wild-type (muscle-derived stem cell); MHC, myosin heavy chain; PM, plating medium.