Figure 4

Propagation of cell chirality with loss of geometric control. Cells preferentially attach to the fibronectin (FN) domain immediately after plating onto alternating fibronectin/polyethylene glycol (PEG) lanes, and they form a biased alignment within the FN domain. With PEG degradation and cell proliferation, the cells migrate out of the FN domain but maintain chirality over the entire surface.