Figure 1

Prelamin A processing, stem cell marker expression, and proliferation potential are affected in Zmpste24 -deficient muscle-derived stem/progenitor cells (MDSPCs). (A) Immunoblot analysis to measure the expression of the C-terminal prelamin A and N-terminal lamin A/C in WT and Zmpste24^-/- MDSPCs relative to β-Actin. (B) RT-PCR to measure expression of stem-cell markers, CD34 and Sca-1, in MDSPC populations (n = three to four cell populations per group). (C) Proliferation of MDSPC populations was measured with a live cell-imaging system for 72 hours. Plotted are averages of normalized cell numbers at each time point calculated from the analysis of three cell populations per group, ± SD. *P < 0.001, Mann-Whitney Rank Sum test.