From: Standardization of human stem cell pluripotency using bioinformatics
Bioinformatic approach | Distinguish iPSCs from ESCs? | Functional assay? | Notes |
---|---|---|---|
No | No unique gene expression profile may be due to small sample size or heterogeneity in iPSC quality. [19, 20, 28] | ||
No | Further exploration of the functional consequences of epigenetic alterations is needed. [33] | ||
Combinatorial profiling (methylation mapping and gene expression signatures) [22, 44] | Yes [22] | No | No functional differences have been linked to the detectible differences in gene expression and DNA methylation used in these studies |
Scorecard profiling (gene expression and epigenetic measures with in vitro differentiation) [22, 45] | Yes | Differentiation propensity was linked to motor neuron differentiation efficiency and functional relevance [22, 45] |