Figure 2

Evidence for differentiation. (A) Compiled data of flow-cytometric analysis during cell culture. The percentage of cells positive for p63, CK5, and CK14 on each day of analysis during the periods of culture is presented. Periods of analysis against the percentage of positive cells for each parameter are marked in the respective axis. Average ± SD of replicate experiments (n = 3), carried out by using different donor PBMNCs are presented. The values are plotted in the bar diagram for comparison of percentage of positive cells on each day. CK14- and CK5-positive cells were absent on day 1. (B) Expressions of p63, CK5, and CK14 genes on the day of starting the culture and after 12 days of culture. GAPDH was the housekeeping gene, and TAp63 was the only KPC-specific gene that amplified on day 1. ΔNp63, CK5, and CK14 were expressed on day 12, and the products showed specific molecular size.