Figure 4

Notch inhibition regulates the fate of SPC-01. (a) Treatment of undifferentiated SPC-01 with the γ-secretase inhibitor DAPT (10 μM) for 48 hours upregulates MASH1 expression in SPC-01. (b, c) On differentiation, cultures of SPC-01 pretreated with DAPT for 48 hours gave rise to a significantly greater proportion of neurons with a CHX10⁺ V2a interneuronal phenotype (P < 0.01, means ± SEM, n = 3). This suggests that differential Notch signaling in the undifferentiated SPC-01 cells is giving rise to subpopulations of ventral interneurons, which can be directed into a V2a interneuronal fate by inhibition of Notch.