Figure 5From: Human umbilical cord mesenchymal stem cell-derived neuron-like cells rescue memory deficits and reduce amyloid-beta deposition in an AβPP/PS1 transgenic mouse modelHUMSC-NC transplantation increased microglial activation. (A) Immunofluorescence staining for microglia with anti-Iba1 in both the hippocampus and cortex. The staining was performed as described in the Methods section. The processed tissue section was stained with rabbit anti-mouse Iba-1 IgG (1:500, Wako), and then visualized with FITC-conjugated secondary antibody. Scale bar represents 20 μm. (B) Quantification of the staining images. Iba-1 burden was calculated as the percentage of Iba-1-positive area over the total area. The image quantification was performed by using the software Image Pro Plus 6 (Media Cybernetics). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; HUMSC-NC-treated group versus PBS-treated group.Back to article page