Figure 6

Flow cytometry analysis of WJ-derived cells cultured in endothelial medium after isolation/expansion in different conditions. A.1 and A.2 - explant- and pellet-derived cells, respectively, belonging to group A, isolated and expanded until P2 in the XF medium, and then subcultured for five consecutive passages in endothelial medium, showed lack of endothelial surface markers; B.1 and B.2 - explant- and pellet-derived cells, respectively belonging to group B, isolated and subcultured for five consecutive passages in endothelial medium, showed differential expression of surface markers: explant-derived cells (B.1) did not gain endothelial surface markers expression, whereas pellet-derived cells (B.2) exhibited CD31, CD105 and CD144 surface markers expression upon culture into the endothelial medium; C. Surface markers expression on enzymatically dissociated, freshly isolated WJ cells (pellet-derived P0); D. Comparative MFI expression of CD44, CD73 and CD90 markers, upon exposure to endothelial differentiation conditions, in: (D.a) explant- versus pellet-derived cells belonging to group A (A), as compared to enzymatically dissociated, freshly isolated WJ cells (pellet-P0); (D.b) explant-derived cells from group B (B) versus explant-derived cells from group A (A). MFI, median fluorescence intensity; P, passage; WJ, Wharton’s jelly; XF, xeno-free.