Figure 3

Validation of selected genes in the cytokine, inflammation, and metalloproteases pathways in MSCs exposed to conditioned medium (CM) from a panel of human cancer cell lines. MSCs were exposed to CM as described in Figure 2, then qRT-PCR was utilized to validate the expression of selected genes in the cytokine, inflammation, and metalloproteases pathways in MSCs exposed to CM of FaDu (a), NCI-H522 (b), MDA-MB-231 (c), MCF7 (d) and PC-3 (e). Data are presented as mean fold change (relative to control MSCs) ± S.D. from at least two independent experiments, n = 4. P values * <0.05, ** <0.005, *** <0.0005. (f) Correlative analysis of the expression of the aforementioned genes in FaDu versus the other cell lines. (g) Quantification of secreted IL1β by ELISA from MSCs exposed to MCF7 or FaDu CM. Data are presented as mean ± S.D. n = 3. MSCs, mesenchymal stem cells.