Figure 1From: Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in ratsFlow cytometric results of cellular apoptosis and Western blot results of oxidative stress. A) Compares the incidence of early (annexin V+/PI-) and late (annexin V+/PI+) phases of apoptosis between healthy adipose-derived mesenchymal stem cells (H-ADMSC) and apoptotic (A) (that is, 96 hours of serum-free cell culture) ADMSC (A-ADMSC). B) For A-ADMSCs, early apoptosis and late apoptosis increased at 24 hours and further increased by 48 hours after re-culture (after 96 hours of serum-free cell culture) in normal culture medium with adequate nutrition. * versus H-ADMSC in early or late phase of cellular apoptosis, P <0.0001. C) DAPI stain (nuclei were stained in blue color) indicated the cell apoptosis (white arrows). Apoptosis (white arrows) in A-ADMSC was markedly increased at 48 hours after being re-cultured in normal culture medium with adequate nutrition. D and E) Oxidized protein in lung (D) and kidney (E) at 72 hours after cecal ligation and puncture (CLP) procedure. DNP, 1–3 dinitrophenylhydrazone; M.W., molecular weight (Note: The right lane and left lane shown on the upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively). 1, sham control (SC); 2, cecal ligation and puncture (CLP); 3, CLP + H-ADMSC; 4, CLP + A-DMSC. * versus other groups with different symbols, P <0.001. Statistical analysis using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡) indicate significant difference (<0.05). ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole.Back to article page