| Gene | Name | Primer sequence 5′ → 3′ | Amplicon size, bp | Ta, °C | Efficiency, % | Correlation |
---|---|---|---|---|---|---|---|
Reference genes | ACTB | Beta actin | CCAGCACGATGAAGATCAAG | 88 | 60 | 98 | 0.998 |
GTGGACAATGAGGCCAGAAT | |||||||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | CAGAACATCATCCCTGCTTC | 187 | 59 | 100 | 0.998 | |
ATGCCTGCTTCACCACCTTC | |||||||
H2A | Histone H2A type 1-C | ATATTCAGGCCGTGCTGCT | 105 | 60 | 100 | 0.999 | |
TTTGGGTTTCAAAGCGTTTC | |||||||
HPRT1 | Hypoxanthine phosphoribosyl-transferase 1 | GGCAAAACAATGCAAACCTT | 163 | 57 | 100 | 0.994 | |
CAAGGGCATATCCTACGACAA | |||||||
SDHA | Succinate dehydrogenase complex, subunit A | TCCATCGCATAAGAGCAAAG | 159 | 59 | 99 | 0.997 | |
GGTGGAACTGAACGAACTCC | |||||||
RPL32 | Ribosomal protein L32 | AGCCATCTACTCGGCGTCA | 149 | 60 | 100 | 0.995 | |
TCCAATGCCTCTGGGTTTC | |||||||
UBC | Ubiquitin C | GCAAGACCATCACCCTGGA | 206 | 60 | 97 | 0.996 | |
CTAACAGCCACCCCTGAGAC | |||||||
TUBA4A | Tubulin, alpha 4a | GCCCTACAACTCCATCCTGA | 78 | 60 | 100 | 0.999 | |
ATGGCTTCATTGTCCACCA | |||||||
Test genes | CD40 | Cluster of differentiation 40 | CAGGAAAGAAACTGGTGAATG | 180 | 62 | 106 | 0.993 |
AAGTGGCGTCTGTTGTTGAG | |||||||
CD80 | Cluster of differentiation 80 | CACCTTCACCGACATCACC | 106 | 62-64 | 102 | 0.995 | |
AGACCCCCTTTCGCTCTTC | |||||||
CD86 | Cluster of differentiation 86 | AGTATAAAGGCCGCACAAGC CCTTGGGTAGATGAGCAGGT | 247 | 63 | 95 | 0.990 | |
HGF | Hepatocyte growth factor | TGCATTCAAGGTCAAGGAGA TTTTGGAATTTGGGAGCAGT | 234 | 63 | 84 | 0.983 | |
IDO | Indoleamine 2,3-dioxygenase | ACAACATCAGGACCAGGACAC TCCAGACGCCTTCATAGAG | 198 | 61-64 | 72 | 0.957 | |
TGFβ | Transforming growth factor-β | GGAATGGCTGTCCTTTGATG CGGAGTGTGTTATCTTTGCTGTC | 120 | 61-64 | 92 | 0.997 | |
TNFα | Tumor necrosis factor-α | GCCTCAGCCTCTTCTCCTTC GGCTTGTCACTTGGGGTTC | 172 | 62-64 | 113 | 0.999 |