Table 1 Reverse transcriptase polymerase chain reaction: primers and conditions

β-Microglobulin

Reverse: 5′-ATCTTCAAACCTCCATGATG-3′

Forward: 5′-ACCCCCACTGAAAAAGATGA-3′

SOX2

Reverse: 5′-GCGCCGCGGCCGGTATTTAT-3′

Forward: 5′-CCGGCGGCAACCAGAAGAACAG-3′

c-KIT

Reverse: 5′-CATACAAGGAGCGGTCAACA-3′

Forward: 5′-GTCTCCACCATCCATCCATC-3′

OCT-4

Reverse: 5′-CCACATCGGCCTGTGTATAT-3′

Forward a: 5′-CTCCTGGAGGGCCAGGAATC-3′

Forward b: 5′-ATGCATGAGTCAGTGAACAG-3′

NOTCH-1

Reverse: 5′-TGGCATCAGCTGGCACTCGTCC-3′

Forward: 5′-CCGGCTGGTCAGGGAAATCGTG-3′

KDR

Reverse: 5′-TTTGTCACTGAGACAGCTTGG-3′

Forward: 5′-TATAGATGGTGTAACCCGGA-3′

PPAR-γ

Reverse: 5′-ACAGTGTATGAGTGAAGGAAT-3′

Forward: 5′-CAGTGTGAATTACAGCAAACC-3′

Osteocalcin

Reverse: 5′-TCAGCCAACTCGTCACAGTC-3′

Forward: 5′-GTGCAGAGTCCAGCAAAGGT-3′

Osteopontin

Reverse: 5′-GTCATGGCTTTCGTTGGACT-3′

Forward: 5′-TTGCAGTGATTTGCTTTTGC-3′

RUNX2

Reverse: 5′-GACTGGCGGGGTGTAAGTAA-3′

Forward: 5′-TCTGGCCTTCCACTCTCAGT-3′

Type II collagen

Reverse: 5′-GGGGGTCCAGGGTTGCCATTG-3′

Forward: 5′-ACGGCGAGAAGGGAGAAGTTG-3′