Figure 2

Confocal microscopy of progerin expression in 12-month-old WT and mLMNA⁺mice. In the ascending aortas in wild type (WT) mice (A), nuclei of endothelial cells (arrowheads) and smooth muscle cells (SMCs) (arrows), as expected, are negative for progerin expression. In mLMNA⁺ mice (B), the endothelial cells (arrowheads) in the ascending aortas are progerin negative, whereas all SMCs (arrows) in the intact region of the media show progerin deposition in their nuclei, mostly at the peripheral margin. A similar pattern of progerin expression is seen in the descending segment of the aortas in mLMNA⁺ mice (C and D); all SMC nuclei (arrows) are progerin positive, but the endothelial cells are negative (arrowheads). The lumen of each vessel is labeled (L). Blue = DAPI, green = progerin, red = SMαA. Scale bar: 25 μm.