Figure 4

Proteomic analysis and mechanotransduction protein expression of ascending aortas in WT and mLMNA⁺mice. (A): Proteomic analysis of the ascending aortas in wild type (WT) and mLMNA⁺ mice. This two-dimensional (2-D) gel image shows protein expression in the ascending aortas of WT (green) and mLMNA⁺ (red) mice at 12 months. Yellow spots indicate commonly expressed proteins. The image suggests relatively greater expression of cytoskeleton (myosin regulatory light polypeptide 9, destrin, cytochrome b-c1 complex, actins) and mechanotransduction-related (vimentin, vinculin, transgelin) proteins in WT relative to mLMNA⁺ mice, and relatively greater expression in mitochondrial enzymes and extracellular matrix components in mLMNA⁺ mice relative to WT mice. A complete list of proteins can be found in (B). (C): Mechanotransduction protein levels in the ascending aorta of 12-month-old WT and mLMNA⁺ mice. When vinculin, transgelin and vimentin bands were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH), the quantity of each protein was reduced in LMNA⁺ mice compared with WT. The level of vimentin was the most significantly reduced.