Figure 2From: Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cellsCellular proliferation and extracellular matrix mineralization of hGMSCs after EMD stimulation. (A) The cellular proliferation of hGMSCs after EMD stimulation for 24 hours and 48 hours, by MTS assay (means ± standard deviations, * and #: significant difference at P <0.05 versus 0 and 25 μg/ml EMD, respectively). (B) The extracellular matrix mineralization, stained with ARS, of hGMSCs after EMD stimulation for up to four weeks. (C) Semi-quantitative measurments of the ARS dye from the cultured cells during the osteogenic differentiation of hGMSCs after the EMD stimulations presented in B (means ± standard deviations, and *: significant difference at P <0.05 versus 0 μg/ml EMD at each observation interval). ARS, Alizarin red S; EMD, enamel matrix derivative; hGMSCs, human gingiva-derived mesenchymal stem cells.Back to article page