Figure 2

Characterisation of PD-MSCs. A) In vitro growth curve of the PD-MSCs derived from P2, P10 and P26 placentas at 6 pd. B) FCM analysis of cell surface markers in five-day culture at 6 pd. A negative sample was incubated with the corresponding isotype. The cytograms relative to P2, P10 and P26 placentas are representative of three different experiments with similar results. C) Levels of the indicated mRNA in PD-MSCs from P2 (dark grey), P10 (black) and P26 (light grey) after five days of culture at 6 pd. The data are reported as the level of mRNA relative to the uncultured placenta and are the means + SD (n = 3). Statistical significance: **P <0.01; ***P <0.001. D) Levels of Oct-3/4 protein in PD-MSCs relative to P2, P10 and P26 placentas compared to iPS. Each lane was loaded with 25 μg proteins from cell lysates and β-actin was used as the control for loading equal amounts of proteins. The experiment was repeated three times showing similar results. Band intensities were measured by densitometry. FCM, flow cytometry; iPS, induced pluripotent stem cells; PD-MSCs, placenta-derived mesenchymal stem cells; pd, population doubling; SD, standard deviation.