Figure 7From: Mesenchymal stem cells ameliorate rhabdomyolysis-induced acute kidney injury via the activation of M2 macrophagesComparison of cell surface markers and inflammatory factor expression in M0, M1 and M2 macrophages. (a) Cell surface marker staining of F4/80 and CD206 expression in M0, M1 and M2 macrophages. RAW264.7 macrophages cultured in normal medium were defined as M0 with significant F4/80 and negative CD206 expression. RAW264.7 macrophages stimulated with LPS were defined as M1 with significant F4/80 and weak CD206 expression. RAW264.7 macrophages stimulated with LPS and co-cultured with MSCs in transwells were defined as M2 with significant expression of F4/80 and CD206. (b) ELISA showed that MSC treatment significantly decreased the levels of the pro-inflammatory cytokines TNF-α and IL-6 and increased the levels of the anti-inflammatory cytokine IL-10 in culture medium. *P <0.05 versus M0, #P <0.05 versus M2, n = 3. (c-d) MSCs increased the percentage of CD206+ IL-10+ cells in RAW264.7 macrophages after co-culture for 72 hours. #P <0.05 versus M0 or M1, n = 3. MSCs, mesenchymal stem cells.Back to article page