Figure 4

Combinatorial transcription factor binding identified the Gfi1 -35 kb regulatory region. Raw ChIP-Seq read data from [50] were transformed into a density plot for each transcription factor and loaded into the UCSC genome browser as custom tracks above the UCSC tracks for gene structure and mammalian homology. A discreet binding event for all ten TFs (Scl/Tal1, Lyl1, Lmo2, Gata2, Runx1, Meis1, Pu.1, Fli1, Erg and Gfi 1b) can be seen in the last intron of the 5' flanking gene, Evi5 (indicated by an asterisk). This region was subsequently shown to drive expression in early haematopoietic cells in transgenic mouse embryos [48].