Figure 1From: The secretome of induced pluripotent stem cells reduces lung fibrosis in part by hepatocyte growth factorImmunophenotypic profiles of induced pluripotent stem cell colonies and differentiation of embryoid bodies. (a) Following viral transfection of fibroblasts and culture for 2 to 3 weeks in embryonic stem cell culture medium, induced pluripotent stem cell (iPSC) colonies stained positive for the pluripotent markers OCT4, Nanog, SSEA4 and TRA-1-81 respectively. (b) Derived iPSCs have the ability to differentiate into the three germ layers in vitro as shown after in vitro differentiation of embryoid bodies: β-tubulin III (ectoderm marker), nestin (endoderm marker) and smooth muscle actin (SMA; mesoderm marker).Back to article page