Figure 4

Wnt5a signaling in dental follicle stem/progenitor cells. (A) Western blot was used to detect phosphorylation-Jun N-terminal kinase (P-Jnk) 1/2 expression of Wnt5a transfected cells and vector control; higher expression of P-Jnk1/2 was observed in Wnt5a transfected cells. (B) Exposure to SP600125 for 3 days, a Jnk inhibitor, attenuated Col1a1, Runx2 and osteocalcin (Ocn) in Wnt5a transfected dental follicle cells relative to vector controls as demonstrated by western blot. (C, D, E, F) Real-time quantitative PCR (Taqman) revealed synergistic effects of Wnt5a when exposed to 100 ng/ml BMP2 for 3 days by upregulating Ocn, Runx2 and Col1a1 mRNA, but not alkaline phosphatase (ALP) activity (n = 3; **P <0.01; ***P <0.001). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, not significant.