Figure 5

Soluble amyloid precursor protein (sAPP) can ameliorate proliferation deficits in Human decidua parietalis placenta stem cells (hdpPSCs) induced by GM6001 (GM). (a) Western blot analysis shows that hdpPSCs treated with GM6001 secrete significantly less sAPP compared with hdpPSCs treated with an inactive control (NC). Actin serves as a protein-loading control. (b) Optical density representation of expression intensity of sAPP in Western blot shown in (a). (c) The number of hdpPSCs counted after 3 days of 1 μM NC, 1 μM GM6001, or 1 μM GM6001 + 10 nM sAPPα treatment after original plating of 1,000 cells per well. (d) hdpPSC proliferation as assayed by total cell number after 3 days in culture is reduced by GM6001 in a dose-dependent manner. Red diamond indicates 1 μM NC, blue diamonds indicate GM6001, green diamond indicates 1 μM GM6001 + 10 nM sAPPα, and dotted line indicates the number of cells originally plated. (e) Dose response of sAPPα effect on proliferation of hdpPSCs after 1 μM GM6001 addition. Red diamond indicates 1 μM NC, blue diamond indicates 1 μM GM6001, and green diamonds indicate recombinant sAPPα. Error bars represent standard error of the mean. *P < 0.05, analysis of variance with post hoc analysis. A.U., arbitrary units.