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Table 1 Flow-cytometric analysis of expanded human MSCs

From: A novel serum-free medium for the expansion of human mesenchymal stem cells

Flow cytometry

SFM

SCM

target

% Positive (flow cytometry)

Negative human MSC markers

CD11b

0.49

0.50

CD34

0.26

1.04

CD45

1.58

1.91

CD79a

0.57

0.90

Positive human MSC markers

CD44

99.14

99.73

CD73

99.38

99.90

CD90

99.51

99.63

CD105

97.95

99.78

  1. Analysis of known positive and negative human MSC surface antigens by flow cytometry for cells expanded in both SFM and SCM for three passages.