Figure 3

Induced pluripotent stem cell clone spontaneous differentiation into cells of embryonic germ layers. Wilms tumor (WT)-induced pluripotent stem (iPS) cell, systemic lupus erythematosus (SLE)-iPS cell and autosomal-dominant polycystic kidney disease (ADPKD)-iPS cell clones were allowed to spontaneously differentiate as embryoid bodies in suspension, followed by adherent culture for 10 to 14 days. Differentiated cells were immunostained for markers of ectoderm, endoderm and mesoderm lineages β-III-tubulin (green), FOXA2 (red) and CD31 (PECAM-1) (green), respectively. Nuclei were stained with 4,6-diamidino-2-phenylindole. All images were obtained at 40× magnification.