Figure 2From: The therapeutic effect of mesenchymal stem cell transplantation in experimental autoimmune encephalomyelitis is mediated by peripheral and central mechanismsIntratechal and intravenous injection of mesenchymal stem cells modulates immune response in central nervous system. Expression of FoxP3 in T cells infiltrating the central nervous system of experimental autoimmune encephalomyelitis affected mice at the peak of disease: (a) at protein level, evaluated by intracellular flow cytometry (FACSCanto II; BD Bioscience, Buccinasco, Milan, Italy); and (b) at mRNA level, evaluated by real-time PCR using a LightCycler® 480 (Roche Applied Science, Indianapolis, IN, USA). (c) IL-17 mRNA expression from infiltrating T cells, analyzed by real-time PCR. *P ≤0.05 Mann-Whitney U test. i.v., intravenous; i.t. intrathecal.Back to article page