Figure 5From: Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serumWestern blot analysis showing differentiation markers of osteogenic, vascular smooth muscle, and adipogenic lineages expressed by BM-derived MSCs cultured in different expansion media. The media are M1 (10% FBS + FGF2), M2 (10% FBS + 5% HPL), M3 (10% HPL), and M4 (5% HPL). The experiments were performed in triplicate, and one representative blot is shown here. Beta-actin expression was used as positive control. Osteogenic proteins studied are calcium-sensing receptor (CaSR) and parathormone receptor (PTHR). Adipogenic protein studied is Leptin. Vascular smooth muscle proteins studied are smooth muscle 22alpha (SM22α) and alpha-smooth muscle actin (ASMA). D0, day 0 (before induction); D14, day 14 (after induction).Back to article page