Fig. 3

Analysis of the proteome of the ASCs by mass spectrometry. a Venn diagrams showing the concurrence in identified proteins in the proteome from the three donors (ASC 12, 21 and 23) exposed to either normoxic or hypoxic preconditioning. b Principal component analysis (PCA) of the proteome fractions in all samples. Shown are PCA score plots of principle components 1 and 2 of the protein abundances as measured in the normoxic and hypoxic samples from all three donors in four biological replicates. Red, ASC 12; green, ASC 21; blue, ASC 23; square, normoxic; cross, hypoxic. c Statistical analysis of the difference between label-free samples of the proteome fraction from hypoxic preconditioned ASC 21 against the proteome fraction from normoxic preconditioned ASC21 with a two-sided t test. The results are visualized by a scatter plot (volcano plot). The –log t test p value is plotted against the t test difference log2 for each protein. The proteins significantly changed between the samples (p < 0.05) are in the right and left upper corners (red). ASC adipose-derived stem cell