Figure 7

Immunofluorescent and immunohistochemical stains of DNA damage cellular markers in renal parenchyma after ischemia–reperfusion. Immunofluorescent (IF) and immunohistochemical (IHC) stains of DNA damage cellular markers in renal parenchyma at 72 hours after ischemia–reperfusion (IR) (n = 8). (A) to (E) Microscopic findings of IF stain (400×) for γH2AX⁺ cells (white arrows) in renal parenchyma of five groups. DAPI counter-staining for nuclei (blue). Scale bars in right lower corner = 20 μm. (F) *P <0.0001 vs. other groups with different symbols; † vs. ‡ vs. ¶, all P <0.05 for inter-group comparisons. (G) to (K) Microscopic findings of IF stain (200×) for proliferating cell nuclear antigen-positive (PCNA⁺) cells (red arrows) in renal parenchyma of five groups. DAPI counter-staining for nuclei (blue). Scale bars in right lower corner = 50 μm. (L) *P <0.0001 vs. other groups with different symbols; † vs. ‡ vs. ¶, all P <0.05 for inter-group comparisons. All statistical analyses performed using one-way analysis of variance followed by Bonferroni multiple comparison post-hoc test. Different symbols (*, †, ‡, ¶) among different groups indicate significance at the 0.05 level. ADMSC, adipose-derived mesenchymal stem cells; CsA, cyclosporine A.