Figure 4
From: Identification and characterization of epithelial cells derived from human ovarian follicular fluid
Real-time PCR analysis of human skin fibroblast, human ovarian cortex, epithelial cells cultured for 1 week, and epithelial cells cultured for 1 month. (A) Gene expression of transcripts for pluripotency markers showed higher expression levels for OCT4, Nanog, TERT, and Sox-2 in earlier epithelial cells, and decreased after 1 month of culturing. Human ovarian cortex also showed the presence of transcripts for pluripotency markers. (B) Transcripts for germ cell markers DAZL, STELLA, BLIMP1, STRA8, VAZA, GDF9, SCP3, ZPA, and ZPC in epithelial cells increased significantly after culturing for 1 month. Comparatively, human ovarian cortex also showed different expression levels of transcripts for germ cell markers. Human skin fibroblast cells served as negative controls, and 18s RNA as a house-keeping control gene was detected in all samples. Data represent mean ± standard error of three independent experiments, *P <0.05, **P <0.01. F, human skin fibroblast; OV, human ovarian cortex; E1, epithelial cells cultured for 1 week; E2, epithelial cells cultured for 1 month.