Characterisation of xeno-free bone marrow mesenchymal stem cells (BMSCs)
. (A) Flow cytometry results show that xeno-free BMSCs were uniformly positive for the mesenchymal stem cell markers CD29, CD90, and CD105 but were negative for the haematopoietic markers CD31 and CD34 and for the leukocyte common antigen CD45. (B) Isolated BMSCs exhibited a spindle-like morphology under light field microscopy. Adipogenic and osteogenic differentiations were detected by Oil Red O staining and by Alizarin Red S staining, respectively, to confirm the multipotency of BMSCs. Scale bar: 50 μm.