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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Mesenchymal stem cell-derived microparticles ameliorate peritubular capillary rarefaction via inhibition of endothelial-mesenchymal transition and decrease tubulointerstitial fibrosis in unilateral ureteral obstruction

Figure 4

Anti-fibrotic effects of KMSC or KMSC-derived MPs in UUO kidneys. Light-field microscopic analysis of Masson’s trichrome–stained sections of UUO kidneys treated with vehicle control, KMSC, KMSC-derived MPs, or MP-RNase (A). Quantitative analysis of tubulointerstitial fibrosis in Masson’s trichrome–stained sections using the Image J program (B, upper panel). Immunohistochemical study for α-SMA expression in UUO kidneys treated with vehicle control, KMSC, KMSC-derived MPs, or MP-RNase (A). Quantitative analysis of tubulointerstitial α-SMA–positive area sections using the Image J program (B, middle panel). Immunohistochemical study for F4/80 positive cells in UUO kidneys treated with vehicle control, KMSC, KMSC-derived MPs, or MP-RNase (A). Quantification analysis of F4/80 positive cells per field (B, lower panel). Ten randomly selected high-power fields were quantified and averaged to obtain the value for each mouse (n = 6 for each experimental group). Results are expressed as the mean ± SE of three different experiments. The Kruskal-Wallis test was performed; * P <0.05 versus vehicle control. Black bar represents 50 μm. KMSC, kidney-derived mesenchymal stem cells; MPs, microparticles; SE, standard error; UUO, unilateral uretal obstruction; α-SMA, α-smooth muscle actin.

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Stem Cell Research & Therapy

ISSN: 1757-6512