Figure 2

MenSCs display high proliferation potential, CFU-F capacity, and migration ability. (A) MenSCs show high proliferation. Proliferation was evaluated using cellular mitochondrial dehydrogenase quantification at different time points. MenSCs showed significantly higher proliferation kinetics with respect to BM-MSCs. (B) CFU-F capacity. Serial dilutions of a defined number of cells were cultured and the potential to form CFU was evaluated. MenSCs significantly generated more CFU than BM-MSCs. (C) CFU-F morphology of mesenchymal stem cells. At 21 days of culture, the CFU were stained with crystal violet to visualize the colonies generated. (D-E) Scratch assay. Confluent monolayers of MenSCs and BM-MSCs were mechanically disrupted with a sterile p10 pipet tip. Images were acquired under a phase-contrast microscope. (D) Panels show representative images of BM-MSCs and MenSCs migration post-scratch assay obtained at different time points (magnification 10x). (E) Graph represents statistical analysis of scratch assay. Values are expressed as mean ± SE (*P ≤0.05; **P ≤0.01). Data shown are representative of multiple replicates. BM-MSCs, bone marrow-derived mesenchymal stem cells; CFU-F, fibroblast colony-forming unit; MenSCs, menstrual-drived stem cells; SE, standard error.