Figure 3

Phenotypical characterization of adipose stem cells from the control, obese and postbariatric surgery ex-obese patients. Adipose stromal/stem cells (ASCs) from the subcutaneous adipose tissue of patients for all conditions showed fibroblastoid cells (A, B, C, phase contrast, 100× magnification). Histograms show the ASC surface expression of CD34 (D, E, F, M, N, O), CD73 (G, H, I, P, Q, R) and CD90 (J, K, L, S, T, U) of the cells cultivated at passage 1 (D to L) and expanded up to passage 3 (M to U). Histograms of the ASCs from the control (D, G, J, M, P, S), obese (E, H, K, N, Q, T) and postbariatric surgery ex-obese (F, I, L, O, R, U) samples are representative of three independent experiments. Gray histogram, negative control; red histogram, expression at passage 1; blue histogram, expression at passage 3. Quantitative results are summarized in Table 1. P values from the analysis of variance tests comparing percentage numbers: *P = 0.038. Ctr, control; ExOB, postbariatric surgery ex-obese; OB, obese; PE, phycoerythrin; PECy5, PE-cyanin 5; PERCP, peridin chlorophyll.