Immunophenotypic analysis of human adipose tissue-derived stem cells. Flow cytometry analysis, where the dot plots represent the cell size and granularity of human adipose tissue-derived stem cells (hASCs) cultured in basal medium with (A) allogeneic human serum (aHS) and (B) fetal bovine serum (FBS). Expression of the selected mesenchymal stem cell, hematopoietic and human leukocyte antigens markers are depicted with representative histograms for (A) aHS cultures and (B) FBS cultures. The gray peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD166, CD105, CD90, CD73, CD44, CD54 and HLA-ABC, and did not express CD45, CD34, CD19 and HLA-DR. (C-D) Bar graphs represent the quantitative analysis of the hASC expression pattern of markers in basal medium supplemented with (C) aHS and (D) FBS.