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Table 1 List of primers used for PCR and for real-time PCR gene expression analyses

From: Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing

Name Primer Sequence Product size GenBank Accession number
RUNX2 Forward 5′-TGGTTAATCCGCAGGTCAC-3′ 70 bp NM_001015051.3
  Reverse 5′-ACTGTGCTGAAGAGGCTGTTTG-3′   
AP Forward 5′-AAAGAAGTAGGAGTGGGCTTTGC-3′ 79 bp NM_001442.2
  Reverse 5′-CCCCATTCACACTGATGATCAT-3′   
Collagen II Forward 5′-AGAGACCTGAACTGGGCAGA-3′ 215 bp NM_006719242.1
  Reverse 5′-TGACACGGAGTAGCACCATC-3′   
SOX2 Forward 5′-TTGCTGCCTCTTTAAGACTAGGA-3′ 75 bp NM_003106.3
  Reverse 5′-CTGGGGTAAACTTCTCTC-3′   
C-MYC Forward 5′-CAGCTGCTTAGACGCTGGATTT-3′ 115 bp NM_002467.4
  Reverse 5′-ACCGAGTCGTAGTCGAGGTCAT-3′   
NANOG Forward 5′-CCAACATCCTGAACCTCAGCTAC-3′ 121 bp NM_024865.2
  Reverse 5′-GCCTTCTGCACACCATT-3′   
OCT4 Forward 5′-CAAAGCAGAAACCCTCGTGC-3′ 171 bp NM_001285987.1
  Reverse 5′-AACCACACTCGGACCACATC-3′   
GAPDH Forward 5′-ACATCAAGAAGGTGGTGAAGCAGG-3′ 123 bp XM_005253678.1
  Reverse 5′-TATCGTCAAAGGTGGAGGAGTGG-3′