Table 1 List of primers used for PCR and for real-time PCR gene expression analyses
Name | Primer | Sequence | Product size | GenBank Accession number |
|---|---|---|---|---|
RUNX2 | Forward | 5′-TGGTTAATCCGCAGGTCAC-3′ | 70 bp | NM_001015051.3 |
Reverse | 5′-ACTGTGCTGAAGAGGCTGTTTG-3′ | |||
AP | Forward | 5′-AAAGAAGTAGGAGTGGGCTTTGC-3′ | 79 bp | NM_001442.2 |
Reverse | 5′-CCCCATTCACACTGATGATCAT-3′ | |||
Collagen II | Forward | 5′-AGAGACCTGAACTGGGCAGA-3′ | 215 bp | NM_006719242.1 |
Reverse | 5′-TGACACGGAGTAGCACCATC-3′ | |||
SOX2 | Forward | 5′-TTGCTGCCTCTTTAAGACTAGGA-3′ | 75 bp | NM_003106.3 |
Reverse | 5′-CTGGGGTAAACTTCTCTC-3′ | |||
C-MYC | Forward | 5′-CAGCTGCTTAGACGCTGGATTT-3′ | 115 bp | NM_002467.4 |
Reverse | 5′-ACCGAGTCGTAGTCGAGGTCAT-3′ | |||
NANOG | Forward | 5′-CCAACATCCTGAACCTCAGCTAC-3′ | 121 bp | NM_024865.2 |
Reverse | 5′-GCCTTCTGCACACCATT-3′ | |||
OCT4 | Forward | 5′-CAAAGCAGAAACCCTCGTGC-3′ | 171 bp | NM_001285987.1 |
Reverse | 5′-AACCACACTCGGACCACATC-3′ | |||
GAPDH | Forward | 5′-ACATCAAGAAGGTGGTGAAGCAGG-3′ | 123 bp | XM_005253678.1 |
Reverse | 5′-TATCGTCAAAGGTGGAGGAGTGG-3′ |