Table 1 Overview of primers used for semi-quantitative reverse transcription-polymerase chain reaction
From: Microencapsulated equine mesenchymal stromal cells promote cutaneous wound healing in vitro
Gene | Abbreviation | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
Succinate dehydrogenase complex, subunit A | SDHA | TCCATCGCATAAGAGCAAAG | GGTGGAACTGAACGAACTCC |
Plasminogen activator urokinase | PLAU | TGTGAGATCACTGGCTTTGG | TGACATTCCTGGTGGGAAAC |
Annexin A2 | ANXA2 | GCAGTGTCTGTCACCTCCAG | CCTCCTTCTTGATGCTCTCC |
Cathepsin K | CTSK | TCCAGAAGGGAAACAAGCAC | TCTTATTCCGAGCCATGAGG |
CC chemokine-2 | CCL2 | GGCTCAGCCAGATGCAATTA | GCTTTCTTGTCCAGCTGCTT |
Metallopeptidase-2 | MMP2 | GAAGGCTGTGTTCTTTGCAG | TCCAGTTAAAGGCAGCATCC |
Syndecan-4 | SDC4 | GCAGCATCTTTGAGAGGACAG | GTTTCTTGCCCAGGTCGTAG |
Metallopeptidase-1 | MMP1 | GCCAAATGGACTTCAAGCTG | TAGGAAAGCCGAAGGATCTG |
Tissue plasminogen activator | PLAT | AGTTCTTGCTGGCTCCTGTC | ATCGGTATGTTCTGCCCAAG |
Interleukin-8 | IL-8 | AGACGCACTCCAAACCTTTC | CAGACCTCAGCTCCGTTGAC |
Metallopeptidase inhibitor-1 | TIMP1 | GCTCCCTGGAACAGTCTGAG | CGTCCACAAGCAATCAGTGTC |
Plasminogen activator inhibitor-1 | PAI1 | CCCTGGAGAGTGAAGTGGAC | CCTGCGATACATGGAGAAGC |
Collagen type 1A1 | COL1 | AAGGACAAGAGGCACGTCTG | GCAGGAAAGTCAGCTGGATG |
Collagen type 3 | COLIII | GGGTATAGCTGGTCCTCGTG | GCGCCTCTTTCTCCTTTAGC |
Fibronectin | Fibronectin | AGGTCGTTACTGTGGGCAAC | TAATGGGAGACGGTGTAGGG |
Syndecan-2 | SDC2 | GACTCAGAAAGGAACGTGGAC | ATAACTCCGCCAGCAATGAC |
CXC chemokine-10 | CxCL10 | GACTCTGAGTGGAACTCAAGGAAT | GTGGCAATGATCTCAACACG |