Figure 4
From: Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration
Level of CXCR4 and adhesion proteins in C2C12 myoblasts. (A) Quantitative RT-PCR analysis of mRNA encoding m-cadherin, ADAM-12, syndecan-4, CD9, CD81, integrin β1 (itgb1), integrin α3 (itga3), integrin α7 (itga7), and integrin α9 (itga9) in control cells, treated with Sdf-1, and transfected with CXCR4 siRNA (siRNA). (B) Western blotting analysis of CXCR4, CD9, and tubulin in control, Sdf-1-treated (Sdf-1) and transfected with CXCR4 siRNA (siRNA) C2C12 myoblasts. (C) Immunolocalisation of CXCR4 and adhesion protein in control, treated with Sdf-1, and transfected with CXCR4 siRNA (siRNA) C2C12 myoblasts. Nuclei, blue; adhesion proteins, green. Bar = 50 μm. CXCR, CXC chemokine receptor; siRNA, small interfering RNA. *P <0.05. Error bars indicate standard deviation.