Figure 2

Acyclic retinoid inhibits clonal expansion and proliferation of hepatic stem cells. (A) Tracing colony expansion of hepatic stem cells (HpSCs) with half maximal inhibitory concentration dose (3.5 mg/L) of acyclic retinoid (ACR) during 7 days of culture. (B) Tracing the proliferation of HpSCs during 7 days of culture treated with ACR (n = 6). (C) The number and size of colonies formed at day 5 of ACR treatment (n = 6). (D) Immunofluorescence for Ki-67 after treatment with ACR, and statistical data of percentage of Ki-67 positive cells (n = 6). Scale bar = 200 μm. Data are shown as means ± SD. Mann–Whitney U-test. *P < 0.05; **P < 0.01.